bromophenol blue dye

PubChem Compound. Please fill out this form to request the QC report. Room temperature in continental US; may vary elsewhere. There are currently no Customer reviews or Questions for ab146339.Please use the links above to contact us or submit feedback about this product. Please visit our frequently asked questions (FAQ) page for more details. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. We do not sell to patients. [7] Bromophenol blue is the substance with the highest known value of Kreft's dichromaticity index. Tracking dye for nucleic acid or protein electrophoresis. Thank you for being a loyal MedChemExpress customer, we are here to assist you as needed. If you have published this work, please enter the PubMed ID. © 1998-2020 Abcam plc. Dangui AZ, et, al. Your browser does not have JavaScript enabled and some parts of this website will not work without it. Please note: All products are "FOR RESEARCH USE ONLY. J Colloid Interface Sci. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. 2019 Mar 22; 540: 148-154. [5] It has also been used as an industrial d 2018 Oct 5; 203: 333-341. Please let us know so that we can cite the reference in this datasheet. Publishing research using ab146339? It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Thanks, your subscription has been confirmed. Reaxys. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. For research use only. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6[1][2]. Nanocellulose for gel electrophoresis. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Solutions of the dye, therefore, are blue. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. Submission failed, please try again later. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Preferential solvation bromophenol blue in water-alcohol binary mixture. Get resources and offers direct to your inbox. DMSO : 50 mg/mL (74.63 mM; Need ultrasonic), Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline, Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution, Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline). Migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. Bromophenol blue (115-39-9) Chemical Names and Synonyms. All rights reserved. All of the co-solvents are provided by MCE. PubChem Compound.,,, "Agarose gel electrophoresis (basic method)", "Quantification of dichromatism: a characteristic of color in transparent materials",, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 20 October 2020, at 16:16. Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. [1]. Products are chemical reagents for research use only and are not intended for human use. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. [2]. As an acid–base indicator, its useful range lies between pH 3.0 and 4.6. Need more advice on solubility, usage and handling? Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Migrates at approximately the same rate as 300-500bp DNA… Bromophenol blue, Tracking dye (CAS 115-39-9) (ab146339) | Abcam Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Store under desiccating conditions. Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol), Concentration (start) × Volume (start) = Concentration (final) × Volume (final), This equation is commonly abbreviated as: C1V1 = C2V2, Bromophenol blueOtherstrackingdyeelectrophoresispHindicatorInhibitorinhibitorinhibit. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. 2018 Oct 5; 203: 333-341. Please try the standard protocols listed below and let us know how you get on. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Please contact us to place your order, or try again later. We have received your request and will respond to you as soon as possible. Guaranteed product quality, expert customer support. Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB,[2] albutest[3]) is used as a pH indicator, an electrophoretic color marker, and a dye. To our knowledge, customised protocols are not required for this product. Nanocellulose for gel electrophoresis. Xylene cyanol and orange G may also be used for this purpose.[6]. - Mechanism of Action & Protocol. Dangui AZ, et, al. At neutral pH, the dye absorbs red light most strongly and transmits blue light. InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H, InChI=1/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H, Brc1cc(cc(Br)c1O)C3(OS(=O)(=O)c2ccccc23)c4cc(Br)c(O)c(Br)c4, Except where otherwise noted, data are given for materials in their. 2019 Mar 22; 540: 148-154. Preferential solvation bromophenol blue in water-alcohol binary mixture.

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